##About SickKids

Dedicated exclusively to children and their families, The Hospital for Sick Children (SickKids) is one of the largest and most respected paediatric healthcare centres in the world. As innovators in child health, we lead and partner to improve the health of children through the integration of healthcare, leading-edge research and education. Our reputation would not have been built - nor could it be maintained - without the skills, knowledge and experience of the extraordinary people who come to work here every day. SickKids is committed to ongoing learning and development, and features a caring and supportive work environment that combines exceptionally high standards of practice.

When you join SickKids, you become part of our community. We share a commitment and determination to fulfill our vision ofHealthier Children. A Better World.

Don't miss out on the opportunity to work alongside the world's best in paediatric healthcare.

##Position Description

Molecular Biology Laboratory Technologist II

The Genetics & Genome Biology program is pleased to invite enthusiastic, ambitious, and passionate individuals to apply to begin or continue to develop their research careers under the supervision ofDr. Chantel Trost, an early career researcher who is striving to establish a strong, competitive, transformative, and inclusive research program in the Peter Gilgan Centre for Research & Learning at the Hospital for Sick Children. The position will be full-time, in-person, and almost entirely wet lab-based.

An outstanding candidate with expertise in supporting laboratory research activities and managing day-to-day operations is strongly desired. Experience in a wide variety of laboratory techniques is preferred, including:

• microbiological techniques

o bacterial cell culturing, phage propagation, cloning, plasmid extraction and purification, PCR, DNA transformation, allelic exchange, reporter assays, genetic screens, spectrophotometry, and agarose gel electrophoresis.

• biochemical procedures

o protein purification techniques (e.g., affinity purification, size-exclusion chromatography, and ion exchange chromatography)

o protein detection and characterization methods (e.g., mass spectrometry, western blotting, SDS-and urea-PAGE, native-PAGE, isothermal titration calorimetry, florescence polarization, and circular dichroism spectroscopy).

Knowledge of structural biology (i.e., software, data reporting, and experience with validation techniques, such as site-directed-mutagenesis) is also desired. Proficiency in microscopy techniques, including transmission electron microscopy and florescence microscopy, will be considered an asset, as will expertise in mammalian cell culture techniques and data analysis. Familiarity with various databases (including GenBank) and bioinformatics tools (including software for promoter identification and cloning) is required.

Here's What You'll Love

• An interdisciplinary environment. This lab will utilize microbiology, biochemistry, bioinformatics, structural biology, and microscopy techniques.
• The opportunity to develop novel screening assays.
• The potential to explore, solve, and describe novel biochemical mechanisms.
• The opportunity to develop or participate in the development of translational therapies and/or treatments with clinical significance.
• The opportunity to lead a group of like-minded, team-oriented, compassionate, and kind individuals with guidance and support from Dr. Chantel Trost.
• This position is eligible for employee benefits coverage including but not limited to; health and dental insurance. The benefits package will be discussed at the time of offer.

Here's What You'll Get To Do

Experimentally:

• Work with a wide variety of CRISPR-Cas systems in various bacterial hosts, beginning withPseudomonas aeruginosaandEscherichia coli.
• Work with a wide range of bacteriophages (viruses that infect bacteria).
• Work with mammalian cell cultures.
• Set up high-throughput library screens in cell- and target-based assay formats.
• Perform data analysis and hypothesis generation/testing.
• Collaborate with scientists specializing in a variety of disciplines.
• Contribute your research findings to institutional events and international conferences and assist with submissions to high-impact, peer-reviewed journals.
• Function mostly independently, with guidance from Dr. Chantel Trost as appropriate.

Operationally:

• Maintain oversight of inventory, including biological stocks and consumable supplies.
• Assist with shipping and receiving laboratory supplies.
• Assist with the maintenance of laboratory equipment and computers.
• Maintain detailed files on laboratory equipment, including records of maintenance and repairs.
• Compile and maintain complete records of experimental data and procedures.
• Establish standard operating procedures.
• Ensure biosafety, chemical, and laboratory safety policies and procedures are being complied with by all staff, students, and volunteers.
• Provide continuity in the lab space and research program.
• Support all members of the research team to the best of your ability with ongoing assistance from Dr. Trost.

Personally:

• Pledge that you and all trainees under your supervision strive to meet the highest standards of research and scientific integrity.
• Agree to act professionally and ethically, and model this behaviour to trainees.
• Help foster diversity, equity, and inclusion practices.

Here's What You'll Need

Required qualifications:

• MSc in Molecular Biology-, Chemistry-, Biology,- Biochemistry-, or Microbiology-related disciplines.
• At minimum, two additional full years of wet-lab experience (not course-based).
• Skills in molecular biology, biochemistry, microbiology, and structural biology.
• Track-record of meticulous laboratory record keeping.
• Quantitative skills
• Experience in high-throughput screens, analyzing experimental data, and interpreting complex results.
• Familiarity with various databases (including GenBank) and bioinformatics tools (including software for promoter identification and cloning).
• Ability to construct simple, informative, and visually appealing figures.
• Excellent oral and written communication skills.
• A commitment to understanding and aiding in the pursuit of equity, diversity & inclusion.

Assets:

• Familiarity with CRISPR-Cas systems and/or anti-CRISPRs.
• Understanding of prokaryotic and eukaryotic genetics.
• Expertise in mammalian cell culture techniques and data analysis.
• Knowledge ofin silicomethods.
• Programming skills.
• Strong publication history.

How to Apply

Please upload your cover letter, CV, and a copy of one representative first-author publication. In your cover letter, please provide contact information for three referees who can attest to your research skills and potential. Please also highlight which skills and experiences you have that match the job description, as outlined above.

**Employment Type:**Full-time temporary 1 year contract

#LI-DNI

##Our Commitment to Diversity

SickKids is committed to championing equity, diversity and inclusion in all that we do, fostering an intentionally inclusive and culturally safe environment that reflects the diversity of the patients, families and communities we serve. Learn more about workplace inclusion.

##Accessibility & Accommodation

If you require accommodation during the application process, please reach out to our aSKHR team. SickKids can provide access and inclusion supports to eligible candidates to support their full engagement during the interview and selection process as well as to ensure candidates are able to perform their duties once successfully hired. If you are invited for an interview and require accommodation, please let us know at the time of your invitation to interview. Information received related to access, inclusion or accommodation will be addressed confidentially.

##How To Apply

Technical difficulties? Email ask.hr@sickkids.ca with a short description of the issues you are experiencing. We will not accept resumes sent to this inbox but we are happy to respond to requests for technical assistance.

Tip:Combine your cover letter and resume intoONEdocument of 20 pages or less as you cannot upload multiple documents as part of your application.

Every application is reviewed by a human recruiter and all hiring decisions are made by people. In some cases, AI-assisted tools are used to help review applications based on job-related qualifications.

All positions posted on the SickKids Hospital's Careers Site represent current vacancies, unless otherwise posted in the job description.