Company Description UHN is Canada’s #1 hospital and the world’s #1 publicly funded hospital. With 10 sites and more than 44,000 TeamUHN members, UHN consists of Toronto General Hospital, Toronto Western Hospital, Princess Margaret Cancer Centre, Toronto Rehabilitation Institute, The Michener Institute of Education and West Park Healthcare Centre. As Canada's top research hospital, the scope of biomedical research and complexity of cases at UHN have made it a national and international source for discovery, education and patient care. UHN has the largest hospital-based research program in Canada, with major research in neurosciences, cardiology, transplantation, oncology, surgical innovation, infectious diseases, genomic medicine and rehabilitation medicine. UHN is a research hospital affiliated with the University of Toronto.

UHN’s vision is to build A Healthier World and it’s only because of the talented and dedicated people who work here that we are continually bringing that vision closer to reality.

www.uhn.ca

Job Description Union: Non-Union

Number of vacancies: 1

Site: Krembil Research Institute

Department: Donald K. Johnson Eye Institute

Reports to: Principal Investigator

Hours: 37.5 hours per week

Shifts: Day

Status: Temporary Full-time

Closing Date: August 15, 2025

Position Summary The Krembil Research Institute at the University Health Network (fully-affiliated with the University of Toronto) has an opening for a temporary full-time, Research Technician II in the laboratory of Dr. Karun Singh. This position is available for a one-year contract with possibility for renewal.

Dr. Karun Singh Is a Senior Scientist At The Donald K. Johnson Eye Institute And Krembil Brain Institute At The University Health Network (UHN). They Are Also An Associate Professor At The University Of Toronto. Dr. Singh Has Expertise In Human Stem Cell Models Of Neurological Disorders. The Laboratory Projects Are Focused On

[1] Developing novel stem cell-based 3D organoid models to study the brain and eye

[2] Integrating omics technologies to understand the molecular basis of neurological and retinal diseases

[3] Discovering new therapeutics to treat neurological and retinal disease

[4] To develop stem cell-based therapies

Duties

• Maintaining cell lines (cultured and primary), including multipotent and pluripotent stem cell cultures, using standard sterile techniques
• Conduct experiments to support ongoing lab research projects (Western blots, qRT-PCR, immunocytochemistry and immunohistochemistry)
• Conduct and assist with in vivo experiments in animal models of disease, as well as necropsy and dissection for primary cell isolation
• Coordinate the collection and organization of patient samples for stem cell derivation
• Support the day-to-day operations of the laboratory, including stock preparation, supplies ordering, and general lab maintenance
• Assist the laboratory manager with biosafety compliance, including biological/chemical inventory management

Qualifications

• At minimum, a Bachelors degree in biological sciences, or equivalent discipline is required
• MSc level candidate is preferred
• At minimum, 3 years of related experience is required
• Prior experience with standard laboratory protocols for cell culture, Western blot, qRT-PCR, DNA electrophoresis and genotyping, molecular cloning
• Prior experience with multipotent and pluripotent stem cell cultures is preferred, but candidates willing to learn will be considered
• Prior experience with primary cell isolation from tissue specimens (animals or human) is an asset
• Prior experience with primary cell isolation from tissue specimens (animals or human) is an asset
• Prior experience with mouse models of disease is an asset
• Viral transduction and genetic engineering / gene-editing (e.g., CRISPR/Cas9) experience is an asset
• RNA sequencing and bioinformatics experience is an asset
• Willing to rapidly learn new techniques and skills with training
• Highly-organized and able to prioritize effectively
• Able to communicate and interact with staff, students, colleagues in a professional manner
• Can work independently with minimal supervision

Additional Information Why Join UHN? In addition to working alongside some of the most talented and inspiring healthcare professionals in the world, UHN offers a wide range of benefits, programs and perks. It is the comprehensiveness of these offerings that makes it a differentiating factor, allowing you to find value where it matters most to you, now and throughout your career at UHN.

• Competitive offer packages
• Government organization and a member of the Healthcare of Ontario Pension Plan (HOOPP https://hoopp.com/)
• Close access to Transit and UHN shuttle service
• A flexible work environment
• Opportunities for development and promotions within a large organization
• Additional perks (multiple corporate discounts including: travel, restaurants, parking, phone plans, auto insurance discounts, on-site gyms, etc.)

Current UHN employees must have successfully completed their probationary period, have a good employee record along with satisfactory attendance in accordance with UHN's attendance management program, to be eligible for consideration.

All applications must be submitted before the posting close date.

UHN uses email to communicate with selected candidates. Please ensure you check your email regularly.

Please be advised that a Criminal Record Check may be required of the successful candidate. Should it be determined that any information provided by a candidate be misleading, inaccurate or incorrect, UHN reserves the right to discontinue with the consideration of their application.

UHN is an equal opportunity employer committed to an inclusive recruitment process and workplace. Requests for accommodation can be made at any stage of the recruitment process. Applicants need to make their requirements known.

We thank all applicants for their interest, however, only those selected for further consideration will be contacted.